Yet Another Chimeric Read Detector for long reads

Using all-against-all read mapping, yacrd performs:

1. computation of pile-up coverage for each read
2. detection of chimeras

Chimera detection is done as follows:

1. for each region where coverage is smaller or equal than min_coverage (default 0), yacrd creates a gap.
2. if there is a gap that starts at a position strictly after the beginning of the read and ends strictly before the end of the read, the read is marked as Chimeric
3. if gaps length of extremity > 0.8 * read length, the read is marked as Not_covered

Rationale

Long read error-correction tools usually detect and also remove chimeras. But it is difficult to isolate or retrieve information from just this step.

DAStrim (from the DASCRUBBER suite does a similar job to yacrd but relies on a different mapping step, and uses different (likely more advanced) heuristics. Yacrd is simpler and easier to use.

Input

Any set of long reads (PacBio, Nanopore, anything that can be given to minimap2 ). yacrd takes the resulting PAF (Pairwise Alignement Format) from minimap2 or MHAP file from some other long reads overlapper as input.

Requirements

• Rust in stable channel

Instalation

With cargo

If you have a rust environment setup you can run :

cargo install yacrd

With conda

yacrd is avaible in bioconda channel

if bioconda channel is setup you can run :

conda install yacrd

From source

``` git clone https://github.com/natir/yacrd.git cd yacrd git checkout v0.3

cargo build cargo test cargo install ```

Usage

1) Run Minimap2: minimap2 reads.fq reads.fq > mapping.paf or any other long reads overlapper. 2)

``` yacrd 0.3 Ninetales Pierre Marijon pierre.marijon@inria.fr Yet Another Chimeric Read Detector

USAGE: yacrd [-i|--input] [-o|--output]

[-f|--filter] file.paf -o mapfile.yacrd yacrd -i mapfile.mhap -o mapfile.yacrd yacrd -i mapfile.xyz -F paf -o mapfile.yacrd yacrd -i mapfile.paf -f sequence.fasta -o mapfile.yacrd zcat mapfile.paf.gz | yacrd -i - -o mapfile.yacrd minimap2 sequence.fasta sequence.fasta | yacrd -o map_file.yacrd --fileterd-suffix _test -f sequence.fastq sequence2.fasta other.fastq Or any combination of this.

FLAGS: -h, --help Prints help information -V, --version Prints version information

OPTIONS: -i, --input Mapping input file in PAF or MHAP format (with .paf or .mhap extension), use - for read standard input (no compression allowed, paf format by default) [default: -] -o, --output

Path where yacrd report are writen, use - for write in standard output same compression as input or use --compression-out [default: -] -f, --filter ... File containing reads that will be filtered (fasta|fastq|mhap|paf), new file are create like {originalpath}fileterd.{original_extension} -F, --format Force the format used [possible values: paf, mhap] -c, --chimeric-threshold Overlap depth threshold below which a gap should be created [default: 0]

-n, --not-covered-threshold <not-covered-threshold>
        Coverage depth threshold above which a read are marked as not covered [default: 0.80]

    --filtered-suffix <filtered-suffix>
        Change the suffix of file generate by filter option [default: _filtered]

-C, --compression-out <compression-out>
        Overlap depth threshold below which a gap should be created [possible values: gzip, bzip2, lzma, no]

```

Output

type_of_read id_in_mapping_file length_of_read length_of_gap,begin_pos_of_gap,end_pos_of_gap;length_of_gap,be…

Example

Not_covered readA 4599 3782,0,3782

Here, readA doesn't have sufficient coverage, there is a zero-coverage region of length 3782bp between positions 0 and 3782.

Chimeric readB 10452 862,1260,2122;3209,4319,7528

Here, readB is chimeric with 2 zero-coverage regions: one between bases 1260 and 2122, another between 3209 and 7528.