perform interval queries on an indexed fasta file
This is a simple utility library to request interval queries on an indexed fasta file.
Ths index is assumed samtools faidx.
The fasta file is memory-mapped and a single mutable buffer is kept for the indexed fasta reader. This buffer is used to return a slice reference to the query sequence but allows for a non-contiguous sequence (i.e. can return sequences without newlines)
Note that because a mutable buffer is kept, this is not the best approach for concurrent operations.
However, for single-threaded applications, this performs very well with low memory overhead and runtime.
```rust use anyhow::Result; use faiquery::{FastaIndex, IndexedFasta};
fn main() -> Result<()> { let index = FastaIndex::fromfilepath("exampledata/example.fa.fai")?; let mut faidx = IndexedFasta::new(index, "example_data/example.fa")?;
// Query the first 10 bases of chr1
let seq = faidx.query("chr1", 0, 10)?;
assert_eq!(seq, b"ACCTACGATC");
// Query the first 10 bases of chr2
let seq = faidx.query("chr2", 0, 10)?;
assert_eq!(seq, b"TTTTGATCGA");
Ok(())
} ```
There are other index fasta readers that are available, here is a nonexhaustive list of those:
If you are looking for a powerful concurrent reader I recommend using the faimm crate.