Generates randomized CAS12 style 6-plex CRISPR reads.
A construct is defined by the following structure:
text
{stagger}{left_adapter}
[{constant}{spacer}] *6
{right_adapter}{stagger}
Where the stagger
is a random sequence of 0-8 basepairs,
the {left,right}_adapter
is not guaranteed to be the
same length.
The constant
region will be the same length and the spacer
region is the same length as well.
bash
git clone https://github.com/noamteyssier/casgen
cd casgen
cargo install --path .
bash
casgen