casgen

Generates randomized CAS12 style 6-plex CRISPR reads.

Construct

A construct is defined by the following structure:

text {stagger}{left_constant} [{spacer}{variable}] *6 {right_constant}{stagger}

Where the stagger is a random sequence of 0-8 basepairs, the {left,right}_constant is not guaranteed to be the same length. The spacer region will be the same length and the variable region is the same length as well.

Results

The following sequences will

Usage

Installation

bash git clone https://github.com/noamteyssier/casgen cd casgen cargo install --path .

Running

bash casgen