Generates randomized CAS12 style 6-plex CRISPR reads.
A construct is defined by the following structure:
text
{stagger}{left_constant}
[{spacer}{variable}] *6
{right_constant}{stagger}
Where the stagger
is a random sequence of 0-8 basepairs,
the {left,right}_constant
is not guaranteed to be the
same length.
The spacer
region will be the same length and the variable
region is the same length as well.
The following sequences will
bash
git clone https://github.com/noamteyssier/casgen
cd casgen
cargo install --path .
bash
casgen